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AFLP-analysis of Asterionella formosa in Dutch and Swiss lakes

Archived data
Availability: Unrestricted
The data are freely available to anybody and may be used for any purpose. Usage acknowledgement may be required

Description
The dataset contains AFLP fingerprinting scores of 160 clonal isolates of the diatom Asterionella fromosa originating from several Dutch and Swiss lakes isolates in the years from 2001 to 2010. The band scores are presented in the sheet "AFLP results", the isolation locations and dates for the Asterionella isolates are presented in sheet "strains and isolation date", more information is included in the metadata. more

This dataset presents the fingerprinting scores of 160 different clonal isolates of the freshwater diatom Asterionella formosa based on amplified fragment length polymorphism (AFLP). As Asterionella colonies consist of uniclonal cells only, isolating single colonies is a simple way to achieve uniclonal cultures. Culture establishment from isolated colonies was fairly unbiased with a larger than 95% success rate. The AFLP analysis was performed by Keygene® (Wageningen, The Netherlands) using the same four EcoRI/Msel AFLP primer combinations as reported in De Bruin et al (2004), a) Eco+GA & Mse+AT, b) Eco+GA & Mse+CC, c) Eco+GA & Mse+CG, d) Eco+GC & Mse+AC. In the AFLP analysis each locus was treated as a separate character and scored for presence or absence, yielding a binary data matrix for each early and late bloom subset of genotypes separately.

Scope
Keywords:
Fresh water, Aflp, Algae, Bacillariophyta, Genetic diversity, Netherlands, Switzerland, Asterionella formosa Hassall, 1850, Fragilaria crotonensis Kitton, 1869

Geographical coverage
Netherlands [Marine Regions]
Switzerland [Marine Regions]

Temporal coverage
2001 - 2010

Taxonomic coverage
Asterionella formosa Hassall, 1850 [WoRMS]
Fragilaria crotonensis Kitton, 1869 [WoRMS]

Parameter
AFLP band scores Methodology
AFLP band scores: AFLP

Contributors
Katholieke Universiteit Leuven; Campus Kortrijk; Faculteit Wetenschappen en Technologie; Departement Biologie; Laboratorium Aquatische Biologiedata creator
Royal Netherlands Academy of Arts and Sciences; Netherlands Institute of Ecology (NIOO-KNAW), moredata creatordata manager

Publication
Based on this dataset
Gsell, A.S. et al. (2013). Chytrid epidemics may increase genetic diversity of a diatom spring-bloom. ISME J. 7(10): 2057-2059. https://hdl.handle.net/10.1038/ismej.2013.73, more
Gsell, A.S. et al. (2012). Genotype-by-temperature interactions may help to maintain clonal diversity in Asterionella formosa (Bacillariophyceae). J. Phycol. 48(5): 1197–1208. dx.doi.org/10.1111/j.1529-8817.2012.01205.x, more

Dataset status: Completed
Data type: Data
Data origin: Research: field survey
Metadatarecord created: 2013-06-03
Information last updated: 2013-06-03
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